Progress in macromolecular crystallography depends on further miniaturization of crystallization experiments
نویسندگان
چکیده
منابع مشابه
Progress in macromolecular crystallography depends on further miniaturization of crystallization experiments.
X-ray crystallography is currently by far the most successful technique for determining the structures of biological macromolecules, their complexes and assemblies to high-resolution (better than 3.5 Å). These structures have been proven to assist the rational design of new drug-like molecules and they are invaluable in helping us to understand life at the atomic level. Nowadays, X-ray crystall...
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The measurement of X-ray diffraction data from macromolecular crystals for the purpose of structure determination is the convergence of two processes: the preparation of diffraction-quality crystal samples on the one hand and the construction and optimization of an X-ray beamline and end station on the other. Like sample preparation, a macromolecular crystallography beamline is geared to obtain...
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Cryogenic cooling of macromolecular crystals is commonly used for X-ray data collection both to reduce crystal damage from radiation and to gather functional information by cryogenically trapping intermediates. However, the cooling process can damage the crystals. Limiting cooling-induced crystal damage often requires cryoprotection strategies, which can involve substantial screening of solutio...
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Modern X-ray structure analysis and advances in high-throughput robotics have allowed a significant increase in the number of conditions screened for a given sample volume. An efficient evaluation of the increased amount of crystallization trials in order to identify successful experiments is now urgently required. A novel approach is presented for the visualization of crystallization experimen...
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CHESS activity report F many years, most synchrotron stations for crystallography, such as CHESS A1, used X-ray sensitive film as the primary detector and xray radiation at about 8 keV (1.5 Å). Two years ago CHESS constructed station F1 on a wiggler line that is tuneable from about 7-14 keV. In practice, this station operates with a standard energy of 0.91 Å. The benefits of using higher energi...
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ژورنال
عنوان ژورنال: Drug Discovery Today
سال: 2014
ISSN: 1359-6446
DOI: 10.1016/j.drudis.2014.07.002